Scientific Program


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09:30 - 10:30 Session I. Cloning and expression
David Friedmann, UC - Producing peptides by the Smt3 expression system
Rhett Kovall, UC - Useful protocols for bacterial expression & protein solubilization
Artem Evdokimov, P&G - Molecular Biology toolbox for crystallography
10:30 - 11:00 Coffee break & discussion
11:00 - 12:00 Session II. Protein purification and characterization
Andrew Herr, UC - Biophysical techniques to aid crystallization
Xu Xing, OSU - A biotinylation/mass spectrometry technique for domain mapping
Jamie Baird-Titus, UC - Practical aspects of fusion protein cleavage with TEV/TVMV protease
12:00 - 13:30 Lunch, discussion, & posters
13:30 - 14:30 Session III. Protein crystallization
Matt Pokross, P&G - New crystallization tools
Long Wang, Purdue - Capping Short DNA Duplex for Protein-DNA Co-crystallization
Ewa Skrzypczak-Jankun, MUO - Flash-freezing crystals of unstable complexes
14:30 - 15:00 Coffee break & discussion
15:30 - 16:00 Session IV. Structure solution & finished structures
Cary Bauer, Bruker - SAD phasing using a home source, resolution extension in SHELX
Maria Szkudlarek, MUO - Structure-based screening of natural urokinase inhibitors
Tom Thompson, UC - Solution of a difficult MIR structure: Follistatin/activin
Richard Walter, P&G - Efficient use of synchrotron facilities, structure solution on the fly
16:00 - 17:00 Panel discussion: Career options in academia, industry & government
 


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