| 09:30 - 10:30 |
Session I. Cloning and expression
|
|
| David Friedmann, UC |
- Producing peptides by the Smt3 expression system |
| Rhett Kovall, UC |
- Useful protocols for bacterial expression & protein solubilization |
| Artem Evdokimov, P&G |
- Molecular Biology toolbox for crystallography |
|
10:30 - 11:00 |
Coffee break & discussion
|
11:00 - 12:00 |
Session II. Protein purification and characterization
|
|
| Andrew Herr, UC |
- Biophysical techniques to aid crystallization |
| Xu Xing, OSU |
- A biotinylation/mass spectrometry technique for domain mapping |
| Jamie Baird-Titus, UC |
- Practical aspects of fusion protein cleavage with TEV/TVMV protease |
|
| 12:00 - 13:30 |
Lunch, discussion, & posters
|
13:30 - 14:30 |
Session III. Protein crystallization
|
|
| Matt Pokross, P&G |
- New crystallization tools |
| Long Wang, Purdue |
- Capping Short DNA Duplex for Protein-DNA Co-crystallization |
| Ewa Skrzypczak-Jankun, MUO |
- Flash-freezing crystals of unstable complexes |
|
| 14:30 - 15:00 |
Coffee break & discussion
|
15:30 - 16:00 |
Session IV. Structure solution & finished structures
|
|
| Cary Bauer, Bruker |
- SAD phasing using a home source, resolution extension in SHELX |
| Maria Szkudlarek, MUO |
- Structure-based screening of natural urokinase inhibitors |
| Tom Thompson, UC |
- Solution of a difficult MIR structure: Follistatin/activin |
| Richard Walter, P&G |
- Efficient use of synchrotron facilities, structure solution on the fly |
|
| 16:00 - 17:00 |
Panel discussion: Career options in academia, industry & government
|
